Stem Cell Colony Assay
Identify single cells, cell clusters, and cluster gaps within stem cell colonies, and quantify cluster area, cell area, gap area, and gap-based cluster classification.
stem cells, phase contrast, cell culture, stem cell colony
The Stem Cell Colony App identifes single cells, cell clusters and cluster gabs (no cells) within cell colonies in culture.The output of the App is total cluster area, total cell area, total cluster gabs area and percentage from cluster area. Further it classifiers the clusters based on number of cluster gabs and percentage from each cluster.
Srinivasan S, Kryza T, Bock N, et al. A PSA SNP associates with cellular function and clinical outcome in men with prostate cancer. Nat Commun. 2024;15(1):9587. Published 2024 Nov 6. doi:10.1038/s41467-024-52472-6
Jyotsna Batra, Queensland University of Technology, Brisbane, Queensland (QLD), Australia
Original Image
Cell detection
Cluster detection
Cluster gap detection
Application Note
14 Oct, 2024
Quantitative Analysis of Cultured Cells: High-End Solution for Ready-To-Publish Data
We support the following file formats:
- TissueFAXS (aqproj)
- StrataFAXS II (vmic)
- PreciPoint (vmic, gtif)
- Generic BigTIFF Import
- Support for multipage BigTIFF files
- OME-TIFF
- JPEG, PNG, BMP, TIFF
- Zeiss (czi)
- Hamamatsu NanoZoomer (ndpi)
- Aperio (svs)
- Leica (scn)
- 3D HISTECH Pannoramic
- Mirax (mrxs)
- Olympus (vsi)
- More slide scanners to be added!
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Custom App development
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You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.
