IF Dots
Detect dots-stainings per cell in up to four markers, segment cellular compartments, measure up to 20 intensity, statistic, and morphometric parameters, and quantify dot count, mean intensity, total area, intensity sum, and per-dot area/intensity per compartment.
cell culture, breast cancer, fluorescence, HER2
The IF Dots App provides dot detection per cell within the cell compartments for up to four markers in a sample (e.g. FISH, RNA). Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters. Dot measurement parameters are provided per cell compartment (e.g. nucleus, cytoplasm) and include count, mean intensity, total dot area, and sum of intensity as well as area and intensity lists for all single dots.
Original image
Nuclei detection
Dot detection
Dot/nuclei detection
Blog Post
15 Feb, 2023
Applications of AI in Cell Segmentation
Application Note
14 Oct, 2024
Quantitative Analysis of Cultured Cells
Webinar
12 Nov, 2025
TG Academy Webinar - Decoding the language of cancer with SCellBOW
Application Note
14 Oct, 2024
Quantitative Analysis of Cultured Cells
We support the following file formats:
- TissueFAXS (aqproj)
- StrataFAXS II (vmic)
- PreciPoint (vmic, gtif)
- Generic BigTIFF Import
- Support for multipage BigTIFF files
- OME-TIFF
- JPEG, PNG, BMP, TIFF
- Zeiss (czi)
- Hamamatsu NanoZoomer (ndpi)
- Aperio (svs)
- Leica (scn)
- 3D HISTECH Pannoramic
- Mirax (mrxs)
- Olympus (vsi)
- More slide scanners to be added!
Related Apps
IF Cardio Cell Culture Dots
Segment cultured cardiac cells, detect cardiomyocytes and fibroblasts, and quantify dot markers (CISH/FISH) per cell, including cell counts and dot number, area, and mean intensity.
cardiology, cardiomyocytes, cell culture, fibroblasts, troponin red, FISH
RNA Scope
Detect nuclei and single-marker dots (CISH/SISH), segment nucleus and/or cytoplasm, measure up to 20 intensity, statistic, and morphometric parameters, and quantify dot count, mean intensity, total area, intensity sum, and per-dot area/intensity per cell.
RNAScope, brightfield, CISH, ISH
Custom App development
Perfectly tailored image analysis solutions for your research.
You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.
