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IF Cardio Cell Culture

Detect nuclei and identify cardiomyocytes in IF-stained cell cultures, reconstruct cytoplasmic masks, and quantify cardiomyocyte number, area, and marker intensity.

cardiology, cardiomyocytes, cell culture, fibroblasts, troponin red

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The IF Cardio Cell Culture App provides cell segmentation, detection of cardiomyocytes (based an appropriate stain e.g. Troponin Red) and fibroblasts within cultured cardio cells plus one additional marker. The App outputs parameters such as number of cardiomyocytes, fibroblasts and markerpos cardiomyocytes, fibroblasts.

Agatha Ribeiro da Silva, Prof. Jose E. Krieger (Heart Institute, University Soa Paulo)

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Original Image

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Nuclei detection

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Cellular mask

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Cardiomyocytes (red), fibroblasts (green)

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Application Note

14 Oct, 2024

Quantitative Analysis of Cultured Cells

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Webinar

12 Nov, 2025

TG Academy Webinar - Decoding the language of cancer with SCellBOW

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Application Note

14 Oct, 2024

Quantitative Analysis of Cultured Cells

We support the following file formats:

  • TissueFAXS (aqproj)
  • StrataFAXS II (vmic)
  • PreciPoint (vmic, gtif)
  • Generic BigTIFF Import
  • Support for multipage BigTIFF files
  • OME-TIFF
  • JPEG, PNG, BMP, TIFF
  • Zeiss (czi)

  • Hamamatsu NanoZoomer (ndpi)
  • Aperio (svs)
  • Leica (scn)
  • 3D HISTECH Pannoramic
  • Mirax (mrxs)
  • Olympus (vsi)
  • More slide scanners to be added!

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IF Cardio Cell Culture Dots

Segment cultured cardiac cells, detect cardiomyocytes and fibroblasts, and quantify dot markers (CISH/FISH) per cell, including cell counts and dot number, area, and mean intensity.

intracellular analysis

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cardiology, cardiomyocytes, cell culture, fibroblasts, troponin red, FISH

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IF Cultured Cells & Substructures

Detect nuclei-based cells, quantify dot markers per cell, and measure cytoskeletal filament density and dot intensity at single-cell level.

intracellular analysis

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FISH, cell culture, cytoskeleton, actin fibres

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IF Cytoskeleton

Detect cytoskeletal structures by specific stain, identify cytoplasm with additional markers, and export filament count (inside, outside, membrane), filament length, and total filament area.

intracellular analysis

single-cell analysis

cell culture

actin, cytoskeleton, cortical fibres, microfilaments, stress fibres, cell culture, fluorescence

Image

Custom App development

Perfectly tailored image analysis solutions for your research.

You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.

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About Us

TissueGnostics provides advanced solutions for whole-slide imaging and image analysis in biological and clinical research. Our products help researchers to scan and analyze complex tissue samples, enabling more detailed insights into tissue structure, cellular interactions, and spatial cell landscape.

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