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SCANNING SOLutions

TissueFAXS Imaging Software

TissueFAXS Imaging Software
Walk-away automation

TissueFAXS is TGs software for the control of the TissueFAXS and TissueFAXS i systems. It provides automated scanning capabilities, a state of the art sample viewer and management of the scan projects.

There are configurations of TissueFAXS for scanning slides, microtiter plates and high-throughput (Slide Loader, 120 slides).

TissueFAXS software is not available as a standalone version or for the control of other microscopes (upgrades of eligible microscopes to TissueFAXS or TissueFAXS S configuration excepted).

TissueFAXS Imaging Software
is integrated into all TG systems

TissueFAXS SL
TissueFAXS SL PLUS
TissueFAXS Spectra
TissueFAXS Q
TissueFAXS PLUS
TissueFAXS Fluo
TissueFAXS Histo
TissueFAXS i PLUS
TissueFAXS i Fluo
TissueFAXS i Histo

TissueFAXS Workflow and Automation

TissueFAXS workflow is based on a "preview scan - tissue or region detection - high magnification scan" approach. Once a solution has been established for a specific batch of slides a profile containing all essential information for scanning this batch of slides can immediately be saved and reused. The profile can very easily be adapted to divergences in the batch.

Scan Flow Chart

TissueFAXS GUI

The TissueFAXS GUI is well organised with tabs and static windows.The Camera and Acquired Images tabs as well as the Preview window can be deployed to become discrete windows, with the camera window usually being deployed to the right screen in the two screen computer setups preferred by TG.

The image on the right shows the standard TissueFAXS GUI with the camera window integrated and visible as a tab behind the sample viewer.

TissueFAXS GUI

TissueFAXS Imaging Software 7.0 & 7.1

The new features of TissueFAXS 7.0 & 7.1 include improvements to the acquisition workflow, quality and visualiz pseudoIHC ation of images, handling of TMAs, and the user interface. Here are some highlights:

7.1 NEW 3D VIEWER for Confocal imaging in TissueFAXS - via right click on a region and from the contextual menu select Region 3D View. Operation shortcuts enable an easy interaction the 3D image.
7.1 For TissueFAXS Spectra: Expanded spectral database for multispectral projects. Predefined markers have been added to make the unmixing process easier.
7.1 Improved Tissue Detection parameters/layout
7.1 Delete individual channels: TissueFAXS allows deleting channel images after regions were scanned.
7.1 Smart Focus Points strategy for focus points distribution uses the preview image to find the best positions in order to avoid low contrast areas, holes and areas outside the tissue plane.
7.0 The Region Viewer Toolbar has been streamlined for more easily navigating and editing images for visualization. Overlay changes can be made by checking or unchecking channels, and a new channel histogram has been added for easier signal detection. Lower and upper thresholds can be set more intuitively and any changes are made to the image in real time.
7.0 The new image compare sets function (Multi Region Viewer) allows to visually compare two or more acquired regions side by side.
7.0 comes with new visualization modes for acquired IF images including Pseudo IHC, providing a pathology view (pseudo coloured images) as well as the HDR-like mode (high dynamic range).
7.0 provides a dedicated database management section for a fast and easy search through all previously acquired projects.
7.0 Improved TMA block visualization and for the TF Spectra configuration, “spectral unmixing” is integrated.
7.0 allows for independent acquisition settings per slide within an experiment. Each individual slide can now contain its own independent acquisition settings for channels, focus, and camera settings within the same experiment.

Tissue Detection Technology, Autofocus, Image Stitching & Illumination Correction

TissueFAXS software in all configurations provides comprehensive and robust automatic tissue detection. To take into account the wide variety of stainings and staining quality as well as the placement and size of tissue sections on the slide the algorithm providing tissue detection has controls for adaptation if needed. There are also drawing tools to add to or subtract from the automatic result to further fine tune. If the user does not need an automatic detection of the whole tissue and prefers to identify specific parts of the tissue visually there also are comprehensive drawing tools to set such regions up for scanning.

Tissue Detection 2017

TissueFAXS systems operate with software autofocus. There are two methods, the focal plane or a defined area with one focus point. The focal plane method has the advantage of having smoother focus transitions between its focal points. The graphic on the right illustrates the focal plane with a simplified virtual overlay. The focal points are shown as small flags. TissueFAXS software also stitches the single field of view images of a sample into a seamless digital slide which is visualised in the softwares. Illumination correction (also known as "vignetting" or "flat field" correction) is available both in brightfield and in fluorescence modes.

TissueFAXS scanning speed

TissueFAXS scanning speed is high for automated microscopy. This is supported on the hardware side by cost-efficient, good quality, fast cameras with large sensors, corresponding llumination systems and SSD drives to rapidly store the images. The TissueFAXS software is permanently optimized to integrate these often changing hardware devices.

The images below exemplify scanning speeds in brightfield and epifluorescence.

1cm2 fluo

Tissue Microarray Imaging

TissueFAXS provides a specialised version of the Tissue Detection algorithm for Tissue Microarray (TMA) scanning. This algorithm recognises TMA blocks on the TMA slide provided that the alleys between the blocks are large enough. Within each recognised TMA block the algorithm also recognises the logical block structure, i.e. how many rows and columns there are in the block. Based on this the algorithm puts placeholders core markers for missing or damaged cores into the block detection. These crosshatched markers will not be scanned, they only serve to keep the nomenclature of the cores intact. Blocks can be moved as a whole, scaled in the x and y axes and rotated. Core markers can be moved and resized either individually, in selected groups or for the whole block.

The images below show some of these operations.

resize tma cores

DAPI 3 layers

Z-STACKING & FISH IMAGING

TissueFAXS also offers Extended Focus scanning (also known as focus stacking, focal plane merging, z-stacking or focus blending) and z-stacks. In this method, a series of images above and/or below the actual focal plane of each field of view are scanned in addition. For each image or channel image this "image stack" is then compressed into one image containing all the areas in focus of the whole image stack, resulting in an optimal image.

This method is e.g. essential for FISH analysis as it ensures that no FISH probe above and beneath the actual focal plane is missed. The z-stack images can optionally be saved to be used in 3D reconstruction.

Cell Culture Vessel Imaging

TissueFAXS also has a dedicated scanning mode for microtiter plates. The most commonly used plate types are stored as templates in the software and can be activated as required. The well regions are then automatically established in the right place for scanning (first image shown below). There also is an inbuilt editor for creating more microtiter plate templates. The well regions are also automatically named. Whole well areas or groups thereof can be resized and moved. There is also an option to draw subregions in a well region (rectangular, spherical or polygonal) and then propagate these into all or a selection of the other well regions. The well regions or their subregions can be scanned and the scans results can then be passed on to TG analysis software to be analysed.

Whole well analysis

High-throughput

TissueFAXS software is also used to scan with the TissueFAXS SL high throughput system in a special configuration allowing the control and management of the 60 two-slide frames (in total 120 slides) in the magazine of the system.

conact bus

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TissueGnostics provides advanced solutions for whole-slide imaging and image analysis in biological and clinical research. Our products help researchers to scan and analyze complex tissue samples, enabling more detailed insights into tissue structure, cellular interactions, and spatial cell landscape.

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