IF Immune Status in situ APP: Distance of tumor cells from blood vessels

The following example was analysed using a combination of the IF Immune Status in situ APP and the FL version of the IHC Angio APP. It was done for the Johns Hopkins Hospital in New York. The aim was to evaluate the distance of tumor cells from blood vessels within the tumor cell clusters.

Tumor areas are detected using cytokeratin⁺ cells (green) and cell density. Blood vessels are detected using CD31⁺ cells (red). The distance of tumor cells from blood vessels within the tumor areas can then be measured. Some basic steps of the analysis are shown below. Final result: Number of tumor cells within each distance range of a blood vessel: 0-50 μm: 6.988/52%; 50-100 μm: 3.483/26%; 100-150 μm: 1.845/14%

Brain tumor whole digital slide