IF Dots APP: FISH

IF Dots APP: FISH

This example shows how the IF Dots APP can be applied to clinical tissue FISH evaluations.

The APP can also be applied to any analysis of dot sized objects, in conjunction with all other StrataQuest features.

For FISH evaluation the recommended workflow is to run the dot algorithm on all dot stainings and the to select suitable nuclei by their large size and high compactness.

 
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Overview of triple staining showing the nucleus 
in DAPI/blue, the housekeeping gene in green and HER2-neu in red.  
DAPI-based segmentation is shown on mouseover.

Selection of suitable nuclei by size and compactness
is shown on mouseover.

 

Detection of green housekeeper gene signal is shown 
in yellow on mouseover. 

                                              

 
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Detection of red HER2-neu signal ishown in yellow on
mousevocer. 
               

Cells with a 3 to 2 dots ratio (only one in this image) are shown by backward gating from the "3 x 2" gate (see below).

 

FISH dot can be displayed and exported in two ways, as shown below:

 Scattergram    Patho table 2

 Scattergrams showing the number of cells with each dot number combination is useful, especially with the Heat Map function enabled which shows those groups with more cells. For the immediate export of data for pathology applications a special table view showing 16 parameters is provided (shown partially on the right side) which offers export to Excel, CSV and pdf forrmats.

 

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