FISH

 

APP for clinical diagnosis of FISH results

Example of FISH analysis:
Overview of triple staining showing the nucleus
in blue, the housekeeping gene in green and Her2-new in red. 
Original image of the Acridin orange channel
and of the insitu hybridization dots.
The sensitivity is adjusted interactively -
only the dots surrounded in red are used.
Her2 new:
Image of Her2 new hybridization products and of recognized dots indicated by the yellow surrounding.
In addition the validation mode helps to clean the result by the use of crops of each cell within the gate and show corresponding overlay as well for fast control.
Validation of results using backward Gating of all cells with two dots per nucleus in case of the housekeeper gene. Cells are recognized are highlighted by the red surrounding line.
 
zh cn