StrataQuest Analysis Software
StrataQuest is TGs most evolved image processing solution for both brightfield and fluorescence images.
It can automatically detect most tissular structures on a digital slide. It integrates detected objects into detailed context-based quantitative analysis.
The software is also an ideal image processing environment for clinicial researchers and dedicated image processors.
There are well over 50 StrataQuest APPs (modules, number constantly growing) which can be used for automated analysis.
StrataQuest is integrated into the following TG analysis systems:
- TissueFAXS Fluo
- TissueFAXS Histo
- TissueFAXS PLUS
- TissueFAXS 120
- TissueFAXS 120 PLUS
- TissueFAXS i Fluo
- TissueFAXS i Histo
- TissueFAXS i PLUS
- TissueFAXS Confocal
StrataQuest is also available as a standalone software.
StrataQuest has importers for the following digital image or slide formats:
- 3D Histech (.mrxs)
- Hamamatsu (.ndpi)
- Zeiss Axio Scan (.czi)
- Yokogawa CQ1 Confocal scanner
- Aperio scanners (.svs)
- Leica (.scn)
- Perkin Elmer (.qptiff)
- Images (.jpeg, .tif, .bmp, .png)
StrataQuest - as easy as you need it, as powerful as you want it...
StrataQuest packs unprecedented analysis performance into a well organized interface.
It also puts it at the fingertips of users who do not have the experience, specialised mathematical know how or simply the time for high level image analysis.
Easy - It is all done with APPs!
A StrataQuest APP is simply an analysis solution for a specific task. It is complete up to the point of providing the specific analysis results ready to be exported to, e.g., Excel or CSV formats.
This is complemented by an export function for all images from the software.
The core feature of an APP is a simplified, tabbed "Easy Mode" user interface. While an APP is meant to be run by simply clicking the "Analyse" button, the interface allows the user to make adaptations to sample/staining variance if necessary.
TGs decade-long experience in providing analysis solutions to the biosciences market has taught that vaunted "only one click" products work - but only on the textbook quality image on which they are presented.
To boldly analyse what has not been analysed before
StrataQuest advanced features allow more experienced users to build their own solutions.
The ideal tool to do this is StrataQuests logically structured Advanced Mode interface and highly adaptable sample viewer.
Solutions created in Advanced Mode can be given an Easy Mode interface and saved as APPs for others to use.
This capability, together with the possibility to plug in Matlab, ImageJ and FIJI solutions also make StrataQuest an ideal tool for imaging core facilities.
StrataQuest provides a special dual purpose feature set.
One purpose is visually sorting and marking specific cell types for , e.g., harvesting in the TissueFAXS SORT system.
The other purpose is detecting and analysing small cell subunits within a larger organ („clusters“in the StrataQuest nomenclature). Clusters are defined by a specific central cell (phenotyped by its staining) and the other cluster cells by distance and optionally also by specific staining.
The image below describes the main parts of both workflows on the basis of a colon sample stained with DAPI and for FOXP3, CD25 and CD4.