Tissue Cytometry

Automated identification and functional characterization of single cells in tissue sections. In contrast to morphometry, which provides values referring to the metric dimensions of cells (area, perimeter, distance, length of structures), the term “tissue cytometry” refers to quantification of molecular parameters.
By using antibody staining (immunohistochemistry, immunofluorescence) the presence and/or amount of certain types of molecules in defined cell populations can be measured and this provides conclusive information about cell types (epithelial cell, lymphocyte, killer cell, tumor cell,…), cellular state (resting, growing, dying, ...), and function (cytokines, signaling molecules,…).
Though methodically different, tissue cytometry exhibits a functional similarity to flow cytometry (instruments doing flow cytometry are also known as FACS – Fluorescence Activated Cell Sorter). While flow cytometer is restricted to cells in suspension (e.g. blood) and cannot be applied to solid tissue, tissue cytometry refers to the cytometric analysis (as opposed to morphometric analysis) of histological sections. TissueGnostics has been the first manufacturer of tissue cytometers offering a flow cytometry-like workflow (but applied to tissue sections). Thus TissueGnostics’ systems are termed “TissueFAXS”, “HistoFAXS”, etc.



TissueFAXS

“TissueFAXS” – Fluorescence

A complete system (hard- & software) for tissue cytometry of immunofluorescence stained tissue sections. The standard configuration comes with a scanning stage for 8 slides; also available as TissueFAXS 200 with slide loader for high throughput scanning of 200 slides. […]


TissueFAXS Automated Acquisition Video



TissueFAXSi plus

“TissueFAXS plus” – Fluorescence and brightfield

A complete system (hard- & software) for tissue cytometry of immunohistochemically and/or immunofluorescence stained tissue sections. The standard configuration comes with a scanning stage for 8 slides; also available as TissueFAXSplus 200 with slide loader for high throughput scanning of 200 slides. […]

Acquisition with Exfo Xcite Xled Video

Highspeed Acquisition Fluorescence Video



TissueFAXSi plus

“TissueFAXS inverted” – Fluorescence and brightfield

A complete system (hard- & software) for cell & tissue cytometry of immunohistochemically and/or immunofluorescence stained tissue sections and adherent cell culture monolayers. This system can also be equipped with a culture chamber to perform live cell imaging, time-lapse microscopy, and time kinetic experiments. The system can work with 4 or 8 slides or 1 micro-well plate (up to 384 wells per plate). […]

TissueFAXSi Wellplates Acquisition Video



HistoFAXS

“HistoFAXS” – Brightfield

A complete system (hard- & software) for tissue cytometry of immunohistochemically stained tissue sections. The standard configuration comes with a scanning stage for 8 slides; also available as HistoFAXS 200 with slide loader for high throughput scanning of 200 slides. […]


Highspeed Acquisition Brightfield Video

TMA Acquisition Video

Bone Marrow Video



HemoFAXS

“HemoFAXS” – Blood Smears

A complete system (hard- & software) for analysis of Gimsa-stained (or similar stain) blood smears. The systems performs a differential blood count and automatically locates white blood cells and classifies the leukocytes using machine learning (SVM - “Support Vector Machine”) technology. The standard configuration comes with a scanning stage for 8 slides; also available as HemoFAXS 200 with slide loader for high throughput scanning of 200 slides. Automated oiling and Optical Character Recognition (OCR) of machine-printed slide labels as well as bar-code reading are included. […]

HemoFAXS Presentation Video



StrataFAXS

“StrataFAXS” – Fluorescence and brightfield

A complete system (hard- & software) for tissue cytometry, morphometry, and analysis of multicellular tissue structures, complex morphological features, and multi-parametric interactions within solid tissue. The system offers a complete image analysis environment (design and/or implementation of custum algorithms) and supports analysis of  immunohistochemically and/or immunofluorescence stained tissue sections.
The standard configuration comes with a scanning stage for 8 slides; also available as StrataFAXS 200 with slide loader for high throughput scanning of 200 slides.


StrataFAXS

“StrataFAXS inverted” – Fluorescence and brightfield

A complete system (hard- & software) for tissue cytometry, morphometry, and analysis of multicellular tissue structures, complex morphological features, and multi-parametric interactions within solid tissue and cell cultures.. The system offers a complete image analysis environment (design and/or implementation of custum algorithms) and supports analysis of  immunohistochemically and/or immunofluorescence stained tissue sections, tissue cultures, or adherent (& even confluent) cell culture monolayers.
The system can work with 4 or 8 slides or 1 micro-well plate (up to 384 wells per plate).