Automated identification and functional characterization of single cells in tissue sections. In contrast to morphometry, which provides values referring to the metric dimensions of cells (area, perimeter, distance, length of structures), the term “tissue cytometry” refers to quantification of molecular parameters.
By using antibody staining (immunohistochemistry, immunofluorescence) the presence and/or amount of certain types of molecules in defined cell populations can be measured and this provides conclusive information about cell types (epithelial cell, lymphocyte, killer cell, tumor cell,…), cellular state (resting, growing, dying, ...), and function (cytokines, signaling molecules,…).
Though methodically different, tissue cytometry exhibits a functional similarity to flow cytometry (instruments doing flow cytometry are also known as FACS – Fluorescence Activated Cell Sorter). While flow cytometer is restricted to cells in suspension (e.g. blood) and cannot be applied to solid tissue, tissue cytometry refers to the cytometric analysis (as opposed to morphometric analysis) of histological sections. TissueGnostics has been the first manufacturer of tissue cytometers offering a flow cytometry-like workflow (but applied to tissue sections). Thus TissueGnostics’ systems are termed “TissueFAXS”, “HistoFAXS”, etc.
A complete system (hard- & software) for tissue cytometry, morphometry, and analysis of multicellular tissue structures, complex morphological features, and multi-parametric interactions within solid tissue and cell cultures.. The system offers a complete image analysis environment (design and/or implementation of custum algorithms) and supports analysis of immunohistochemically and/or immunofluorescence stained tissue sections, tissue cultures, or adherent (& even confluent) cell culture monolayers.
The system can work with 4 or 8 slides or 1 micro-well plate (up to 384 wells per plate).[…]
Based on upright or inverted microscope.