Infliximab induces downregulation of the IL-12/IL-23 axis in 6-sulfo-LacNac (slan)1 dendritic cells and macrophages
Dr Brunner from the Medical University of Vienna, Department of Dermatology used TissueFAXS and TissueQuest to investigate the effect of anti-TNF-alpha antagonist therapy infliximab in patients with psoriasis.
Dr Brunner and his group reported that up to 48 hours after the intravenous administration of the TNFalpha antagonist infliximab, they encountered no overt changes in numbers of TNF-a1 cells or signs of apoptosis in lesional psoriatic skin. Yet they observed a rapid decrease in IL-12p40, IL-1b, CCL20, and IL12RB1 mRNA levels. They concluded that the decrease in tissue inflammation during anti–TNF-alpha therapy is not due to immediate killing of TNF-alpha–producing cells but rather results from a rapid downregulation of the pathogenic IL-12/IL-23–driven immune response.
(J Allergy Clin Immunol 2013)
Dear valued distribution partners around the globe,
TissueGnostics is proud to announce that we now offer two new devices that complement our TissueFAXS/HistoFAXS as well as HemoFAXS product lines:
1. Cell Stainer to automate immunological staining procedures and thereby greatly improve staining quality! Needless to mention that an improved staining quality will also create an improved quality of analytical data in our image analysis applications (HemoFAXS, TissueQuest, HistoQuest, StrataQuest)
2. Cellspin centrifuge for harvesting cells in body fluidsCellspin centrifuges PDF Sheet
Cellstain stainers PDF Sheet
Clinical Laboratory of Vetsuisse, Faculty of the University of Zurich and TissueGnostics cooperation
TissueGnostics hereby announces the success of a cooperation with Prof. Dr. Regina Hofmann-Lehmann and Dr. Barbara Riond at the Clinical Laboratory of Vetsuisse Faculty of the University of Zurich, Switzerland in the field of hematology. Aim of the cooperation was to development a HemoFAXS for veterinary medicine and to train leukocyte classifiers for cat, horse and canine species. The results of a comparison study of HemoFAXS versus manual counting when analyzing canine blood smears have been summarized in an abstract with the title:
EVALUATION OF THE HEMOFAXS AUTOMATED DIGITAL WHITE BLOOD CELL DIFFERENTIATION SYSTEM FOR ITS USE IN THE CANINE SPECIES by
Barbara Riond, Martina Stirn, Vivien E. Rampling, Regina Hofmann-Lehmann Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Switzerland which will be presented at a conference in Berlin.
The HemoFAXS development teams of TissueGnostics in Vienna and Iasi congratulate!
Vetsuisse Fakultät, Universität Zürich
TissueQuest employment for the optimization of Nanoparticle quantitative tracking methods
The Department of Biochemistry and Molecular Pharmacology at the “IRCCS- Mario Negri Institute for Pharmacological Research" is a worldwide leader group in the field of pharmacokinetic and, in the last few years, it focused its attention of the fate of Nanotechnologies products (both in pharmacology and toxicology) in vitro and in vivo models. In July 2007 the Institute moved to a new building where a "mouse clinic" has been set up. To be competitive in this constantly evolving field, the mouse clinic was equippedby modern instruments for experiments of in vivo imaging in rodents ...Read More
TissueGnostics is happy to inform you that the reference publication 105 has been published
y-Gamma-Secretase Inhibitors Prevent Overgrowth of Transplanted Neural Progenitors Derived from Human-Induced Pluripotent Stem Cells
It stems from the "Center for iPS Cell Research and Application, Kyoo University”. The head of this institute is Nobel prize laureate Dr. Yamanaka!
20.06.2013- TissueGnostics distributes now the Galileo TMA CK Family
The TMA technology is a technical procedure that combines tens to hundreds of paraffin-embedded tissue specimens into a single paraffinblock. The use of relatively small tissue cores, positioned onto a unique slide, allows simultaneous histological analysis of hundreds of samples.
19.06.2013- TissueGnostics announces the launch of a New Software Suite called "StrataQuest"
TissueQuest 4.0 and HistoQuest 4.0 are the current state-of-the-art solutions for cellular analysis of tissue sections and adherent cell culture monolayers in similarity to flow cytometry.
Starting with the next product generation there will be a whole new dimension provided by our world-leading analysis applications for tissue cytometry - i.e. the ability to analyze "cellular meta-structures" or tissue formations that consist of multiple/many cells! This new product will be termed "StrataQuest".
The new TissueQuest-Strata and HistoQuest-Strata will be available for cellular analysis and meta-structure analysis!
05.06.2013- HistoQuest 3.5 and TissueQuest 4.0 analysis software give users the possibility to import and analyze images and projects generated by different types of scanners.
In case of HistoQuest 3.5 also the TissueFAXS TMA tool has been integrated into the analysis software.
- Import TissueFAXS and HistoFAXS projects
- Import projects from Hamamatsu scanner Nonozoomer
- Import projects from Mirax scanner from 3DHistec
- Import projects from Pannoramic scanner from 3DHistec
- Import projects acquired with AxioScan.Z1 from Carl Zeiss
- Import projects from Aperio ScanScope
- Import projects from Leica SN 400
05.05.2013- Automated microscopy; evaluation of the DM96 and the HemoFAXS
In 2004, the Cellavision DM96 digital microscope was introduced. Recently, TissueGnostics introduced the Hemofaxs. An automated microscope that has a comparable continuous mode to the DM96, but, in addition, is a self-learning system that combines the advantages of programmed and human intelligence offering a tailor-made cell classification for each customer.
05.04.2013- New X-Cite XLED1 speeds up fluorescence scanning.
The integration of the new X-Cite XLED1 speeds up fluorescence scanning by offering high speed switching between four different wavelengths.
This speed boost of an extra 40% can be further increased by the use of a multi-bandpass filter which is integrated into the TissueFAXS workflow.
Watch the X-Cite XLED1 video here:
20.03.2013- Brightfield and fluorescence high speed scanning
Using new, faster cameras, improved microscope control software and SSD disks, we have radically lowered scanning times both for brightfield and fluorescence samples on our TissueFAXS and HistoFAXS scanners.
Scans in brightfield can now be as fast as 47 seconds for 1 cm² using the Baumer HxG 40 c camera with an 1x adapter.
In Fluorescence, 1 cm² with 4 channels at 20x, using a 1x adapter, can be scanned in: 23 minutes, 3 seconds with the PCO Pixelfly USB at 14 Bit.
7 minutes, 2 seconds with the Hamamatsu Orca Flash 4.0 at 16 Bit.
23.11.2012– Imatinib May Help Treat Aggressive Lymphoma
31.10.2012- A new paper using the TissueFAXS System was just accepted in Clinical Cancer Research.
This study establishes the feasibility of detecting BCR activation in primary FFPE biopsy specimens of DLBCL. It lays a foundation for future dissection of signal transduction networks in DLBCL and provides a potential platform for evaluating individual tumors in patients receiving novel therapies targeting the BCR pathway.
14.10.2012– Neue Krebs-Therapie erfolgreich von Maus auf Mensch übertragen
Wissenschaftler am Ludwig Boltzmann Institut für Krebsforschung (LBI CR) und am Klinischen Institut für Pathologie der Medizinischen Universität Wien (AKH) unter der Leitung von Prof. Lukas Kenner untersuchten einen besonders aggressiven Tumor, das anaplastische großzellige Non-Hodgkin Lymphom (anaplastic large cell lymphoma – ALCL). Unter Verwendung eines genetischen Modells, das diese Erkrankung in der Maus exakt widerspiegelt konnten sie die zentrale Rolle des Schaltermoleküls PDGFR identifizieren. Ihre Ergebnisse ermöglichten dem Team um Kenner einen todkranken Patienten im Endstadium ALCL mit einem spezifischen PDGFR-Inhibitor zu behandeln. Innerhalb von nur 10 Tagen war der 27-jährige Patient tumorfrei, und führt seit mehr als 22 Monaten ein vollkommen normales Leben. Ohne diese Behandlung wäre der Patient vermutlich verstorben. Diese Ergebnisse wurden kürzlich in der renommierten Zeitschrift „Nature Medicine“ veröffentlicht.
Die Forschung wurde in Kooperation mit den folgenden Forschunginstitutionen durchgeführt: Harvard Medical School, New York University School of Medicine, University of Bologna, University of Edinburgh, University of Torino, Center for Molecular Medicine of the Austrian Academy of Sciences, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Ludwig Boltzmann Cluster Oncology, Medizinische Universität Graz, Medizinische Universität Wien, Paracelsus Medizinische Universität in Salzburg, Veterinärmedizische Universität Wien, Research Institute of Molecular Pathology. Entscheidend für Arbeit war auch die HistoQuant Technologie der Firma TissueGnostics, mit der Tumoren auf ihre molekularen Eigenschaften quantitativ untersucht wurden.
11.6.2012 – Using extended focus in TissueQuest 4.0 for RNA Dot analysis
The above presentation features one of the major gadgets, that TissueQuest 4.0 offers. By merging different z-Level images, thus creating one sharp layer image, RNA Dot analysis can be carried out quite simply and elegant.For more information regarding z-stacking and/or RNA-analysis please contact: email@example.com
16.5.2012 – HemoFAXS 2.0 with automated oiling and OCR at ISLH 2012“With a broad customer base all over Europe HemoFAXS has become a major contributor to the TissueGnostics product line-up. As a natural consequence we put a lot of effort into developing convenient systems for Hematology labs. The latest evolutionary step of HemoFaxs was to add automation in oiling and OCR (Optical Character Recognition), which is automated Slide-ID reading. Lab staff now only has to insert slides and press scan, which is far less time consuming than the traditional way of blood smear scanning”, says Ilyas Merl (Junior Marketing Manager, TissueGnostics) At the ISLH 2012 Congress, held in Nice (France) from 21st-24th of May, TissueGnostics launches its latest generation of Blood Analysis Systems, the HemoFAXS 2.0. Besides a magnificent increase in reliability, its major improvement is a fully automated oil dispenser, which uses the optimal amount of oil for each slide, in order to get the best image-quality from the 100x magnification objective.
Another step towards full automation was the implementation of an OCR-Device. A small camera at the top end of the microscope now takes pictures of the slide labels and recognizes automatically the slide ID of the blood smear. The ID enables HemoFAXS to get Patient Name, Ward, etc. from the LIS to make data management reporting easy. This all happens automatically.
“HemoFAXS now competes with market leaders. It is a fully functional, automated Hematology System. Boxed systems have a clear disadvantage in price, accessibility and usability. HemoFAXS is also a high-end microscope, fully functional for all major tasks microscopes need to perform”, says Georg Steiner (CEO. TissueGnostics.)
TissueGnostics will display the new HemoFAXS at booth 8/9 at the ISLH in Nice, France, held from 21st – 24th of May. Please visit the booth in the announced exhibition hours (http://www.islh.org/ISLH_2012/). If you can’t make it to Nice, please contact firstname.lastname@example.org for more information.
12.4.2012 – A new Vista on Disease PatternsOn the 12th of April the Symposium “Bridges in Pathophysiology” was held at the Medical University in Vienna. In the context of this meeting the completion of the interdisciplinary project “A new Vista on Disease Patterns” (FFG-project 818 094) was celebrated by different talks of experts from the Medical University in Vienna, Seewald Solutions and TissueGnostics. The Project “A new Vista on Disease Patterns” focused on the development of new algorithms for automated cell and tissue analysis. This cooperation of corporate developers (TissueGnostics and Seewald Solutions) and researchers from the Medical University of Vienna resulted in a greater understanding and appreciation for each other, and at the same time reached its goal of developing algorithms at the very edge of technology.
“The principle investigators Drs. Giovanna Bises, Theresia Thalhammer and Isabella Ellinger from the IPA/MedUniWien in collaboration with the researcher Dr. Alexander K. Seewald (Seewald Solutions) and TissueGnostics GmbH have aimed and succeeded to develop new algorithms for automated segmentation of cells that lack nuclei (erythrocytes), of multinucleated cells (Osteoclasts in culture and placental syncytiotrophoblast), of epithelial and stromal areas within colorectal tumor tissue and of the plasma membrane compartments of cells in the tissue context in this FFG and TissueGnostics-funded Bridge-project. Overall, the intention of this project was to adopt the TissueQuest software for new research challenges, allowing a broad application of the system within many basic and clinical research projects.”, says Ao. Univ. Prof. Dr. - Isabella Ellinger from the Department of Pathophysiology and Allergy Research (IPA) at the Medical University Vienna.
“With the newly developed algorithms integrated into HistoQuest and TissueQuest software, a substantially increase of the performance of our software will occur. Analysis of multi-nucleated cells or cells without nucleus will become simple and elevate the possibilities TissueGnostics offers to researchers dramatically, especially in the field of cancer research”, says Ilyas Merl, Junior Marketing Manager at TissueGnostics.
Dr. Ellinger explains: “This fruitful interdisciplinary project has resulted in the development of various algorithms based on classical image processing and machine learning techniques. This was enabled by the constant support and rapid technical adoption of the TissueFAXS hard- and software components upon any request of the developing team. By that, TissueGnostics GmbH proved to be a reliable and supportive partner for research projects. Apart from the new algorithms developed, the combined efforts of the project partners has so fare enabled publications of seven research articles, more than 17 contributions to national and international symposia and the training of 12 students, among them two PhD students involved in PhD programs of the Medical University Vienna. Not surprisingly, therefore, the professional collaboration within this team has been awarded “with one of the ‘Science2 business Awards’ in the year 2011.”, says Dr. Ellinger.
One of TissueGnostics’ core values is to stay close to researchers in order to implement their expertise into user-friendly and professional tools for research. The cooperation with excellent researchers, like the ones from the Medical University Vienna shows that this principle is and will be a very fruitful contribution to TissueGnostics’ development. “The work contributed by the Medical University Vienna is very valuable and crucial to the success of this project. These experts made it possible for us to gain insights into research, that only experts in the field have. We are looking forward to work with them again”, says Georg Steiner (CEO of TissueGnostics).
01.02.2012, Österreichische Medtech-Firma schreibt Geschichte des digitale Imagings neuDr. Alexandra C. Gruber talks with Katja Österreicher. Read article (German)
11.1.2012, New offices for TissueGnostics Romania and TissueGnostics USAA strong beginning for 2012 as TissueGnostics Romania and TissueGnostics USA moved to new offices. TissueGnostics ties a high level of customer satisfaction to a high level of working place comfort for its employees.
TissueGnostics Romania now accommodates a fully equipped support center and a development core unit which enforces development progress for all the TissueGnostics product lines. “Creating a specific process oriented department with the needed working environment is always a benefit for product quality and innovation”, says Georg Steiner (CEO, TissueGnostics GmbH).
TissueGnostics Support will be available world-wide and fully functional also on holidays, to give customers at all times the help they need. The support team can be reached directly at:
- USA: Tel. +1 (323) 8434024
- Europe: +40 (771) 309792
The TissueGnostics USA sales and support unit moved to its new offices at:
- TissueGnostics USA Ltd.
- 18460 Clark Street
- Tarzana, CA 91356
20.12.2011, Merry Christmas and a happy new year!!!Another year is over. A new one is about to begin. TissueGnostics says thank you to all partners, customers and friends for the fantastic year 2011. We are eager to start into 2012, which will feature several improvements of our current systems and the launch of HemoFAXS 2.0, as well as the continuation of our successful workshop-tour throughout Europe and the participation in several congresses worldwide. Let the next year roll on!
4-7-11.2011, 8th NCRI Cancer Conference, Liverpool, UK“Cancer research in Great Britain seems to have a tremendous need for software tools that simplify workflows and maintain quality levels, which ensure progress in cancer research endeavors. That is exactly where TissueGnostics fits in”, says Arndt Stenzel (Sales Representative, TissueGnostics GmbH). The 8th NCRI Cancer Conference, held in the BT Convention Center in Liverpool, turned out to be a great success for TissueGnostics. Especially the software modules HistoQuest (brightfield) and TissueQuest (fluorescence), for in depth analysis of cultivated cells and tissue , attracted a multitude of scientists and technicians.
At the NCRI conference, the new booth design was introduced. A big HD-screen in the very center , combined with a TissueFAXS plus microscope system and a HistoQuest/TissueQuest workstation offered hands-on experience for interested visitors. “The big screen gives life to the booth, as it displays what can be normally seen only on the small screens at the workstation. This way people passing, or larger groups enjoy a comfortable view on software features.
They don’t watch useless advertisement images. They can see how our software works and then decide whether it is interesting for them” (Georg Steiner, CEO, TissueGnostics GmbH) For more information on congresses and shows attended by TissueGnostics please visit: Congress
6.5.2011 - Phoenix, ArizonaAt the 71st Annual Meeting of the Society for Investigative Dermatology Georg Steiner (CEO of TissueGnostics USA Ltd.) was invited to participate as a lecturer at the Confocal Microscopy and Imaging Techniques-Workshop.
His 20 min presentation featured a wide spectrum of issues concerning image processing from staining to the actual analysis and result interpretation.
Mr. Steiner used HistoQuest Software to analyse a tissue sample with Langerin positive cells and TissueQuest Software for a fluorescence skin sample (UV Irradiation of Skin) in order to cover the principles of:
- 1. Correct staining protocols
- 2. Acquisition of images
- 3. Shading correction
- 4. Definition of Regions of Interest (ROI)
- 5. Color separation
- 6. Segmentation
- 7. Measurement of various parameters
- 8. Comparison of data
“Discussing these essential steps with an audience of scientists, who will definitely use the information given to them, and showing them the capabilities and user friendliness of our TissueGnostics products, was a great experience which already has borne fruits, as we got in contact with people benefiting from our analysis tools”, says Georg Steiner.
If you are interested in the presentation or want to have a web-presentation of the lecture, or if you have any questions on TissueQuest-/HistoQuest Software please contact: email@example.com
We would also like to thank Dr. Christine Bangert (Department of Dermatology, Medical University Vienna) for providing the TissueFAXS data used in this presentation.
04.05.2011 - Using HistoQuest for scientific publicationsTissueGnostics puts a lot of effort into making its products simple to use but at the same time providing the necessary tools for state-of-the-art scientific research.
The latest publication: Expression of organic anion-transporting polypeptides 1B3, 1B1, and 1A2 in human pancreatic cancer reveals a new class of potential therapeutic targets. (Kounnis V, Ioachim E, Svoboda M, Tzakos A, Sainis I, Thalhammer T, Steiner G, Briasoulis E) is already Nr. 53 of the official publication list of TissueGnostics. Download Full Publication List
HistoQuest was successfully used in order to quantify expression of organic anion-transporting polypeptides (OATP) 1A2, 1B1 and 1B1/1B3. Conclusion was that because of the high expression of OATPs 1A1, 1B1 and 1B3 in pancreatic adenocarcinoma further research efforts toward novel therapeutics targeting OATPs is justified. (For full abstract please visit: http://www.dovepress.com/expression-of-organic-anion-transporting-polypeptides-1b3-1b1-and-1a2--peer-reviewed-article-OTT)
“In this publication, HistoQuest is used in a proper way to compare antigen density. The presentation clearly indicates what is measured, and where the density is measured. It also shows that HistoQuest provides all necessary tools and features, combined with high quality graphic support material, to produce “ready-for-publication” output!”, (Georg Steiner, CEO TissueGnostics)
27.02.-03.03.2011 -TissueGnostics at the 100th Annual Meeting of the United States & Canadian Academy of Pathology100 years of expertise in Pathology for the United States and Canada. 100 years of annual meetings of the United States and Canadian Academy of Pathology. For the 100th Meeting TissueGnostics went in for a special treat. Matthew Tolmachoff and Georg Steiner (CEO) put up an 18 sqm booth, including a TissueFAXSi plus system and a workstation equipped with TissueQuest and HistoQuest. Displaying the innovative solutions for cell and tissue analysis to an audience of hundreds of experts from all over the world resulted in many interesting conversations and new contacts..
“We had a very productive time, while talking to our current and our future customers. Talking to these scientists at this conference is always a great experience, as all experts in the field of Pathology join together at this meeting.”, Georg Steiner (CEO TissueGnostics)
There was also some sweat on set, as some of the booth equipment arrived late, making building up time quite short. However, it all paid of.
The big booth and the products presented combined with lots of interested scientist made USCAP meeting 2011 a full success. You will find TissueGnostics in 2012 at USCAP again.
For a list of our planned and past congresses please press here.
08.03.2011 -International Women’s DayInternational Women’s Day: TissueGnostics’ authorized officer Katja Österreicher at Austrian Federal Minister of Science and Research Beatrix Karl for International Women’s day.
“Highly qualified women are essential for innovation. We have to tap the potential of women working at the crossroads of Science and Economy even stronger in the future, and make it visible”, is the Minister convinced. This is also a part of economical recovery, growth and the creation of new high quality jobs.
Together with 10 women being experts in the field of Science- and Technology-Transfer-Business, Katja Österreicher (Authorized officer at TissueGnostics Austria) was invited to participate at a breakfast and a group discussion at the Austrian Ministry for Science and Research in Vienna. The discussion topic was “Strenghtening women in Science and Technology-Transfer” The current situation and challenges and solutions for the support of women in Science and Research were thoroughly discussed.
“The passing on of personal experience is really important, in order to strengthen women and fully intergrate them into Science-, Economy- and Innovations-politics!”, was the conclusion of Beatrix Karl after the meeting.
Katja Österreicher is co-founder of TissueGnostics GmbH and currently holds the position of an authorized officer at TissueGnostics. She is also mother of a 3 year old daughter.
Link to the Article; Austrian Ministry of Science and Research:
01 - 03.12.2010
Global TissueGnostics Distributor Meeting 2010
This year the Global TissueGnostics (TG) Distributor Meeting 2010 added to this experience as 17 participants from Japan, China, Korea, Singapur, Taiwan, Australia, France, Switzerland, Czech Republic, Slowakia, Lithuania, Turkey, UK and Ireland, visited the TissueGnostics (TG) headoffice in Vienna for 3 days of special training.
TG-CEO Dr. Rupert Ecker introduced the distributors not only to each other but encouraged them to share experiences and give feedback on their endeavours. TG-staff mingled with the global TG-distributors and shared the latest developments on HistoQuest- and TissueQuest softwares. “On the whole the purpose was to get together as much experiences and information and start off a global network for the benefit of TG-customers around the world.”, says Dr. Ecker, ”Customers will see a difference as problems will be addressed earlier and solved faster as user feedback is a major input for our current and future software-development.”
Jörg Schlegel from BioInnovas AG (Switzerland), the newest member of the TG-distributiors family also acknowledged: “For me it was very important to get to know the other distributors, especially the Taiwanese, as they have been successful for quite some time.” BioInnovas and TissueGnostics announced partnership for the Swiss area at the beginning of December 2010.
The strong training sessions during the day were followed by visits to traditional Christmas markets and typical Viennese restaurants. All participants enjoyed themselves and prepared for a strong and successful year 2011. Let the new year roll on…
22 - 24.8.2010
TissueGnostics at 14th International Congress of Immunology (ICI) in Kobe
„I am happy to announce that this was the most successfull congress, I have ever attended.“ Rupert Ecker, CEO, TissueGnostics GmbH
From 22nd to 24th of August the 14th International Congress of Immunology took place in Kobe, Japan.TissueGnostic`s CEO Rupert Ecker, together with TG distributors from Japan, Korea, Taiwan and China were highly motivated for the exhibition at the Kobe International Exhibition Hall with its 6000 participants.
However, even their most optimistic expectations were exceeded. Equiped with 10 different types of product folders in Japanese, Korean, Mandarin, Traditional Chinese and English and with 12 exhibitors at the booth the TG team was constantly doing their best to inform, present and explain. „Already on the 2nd day the English folders ran out, fortunately we had some bi-lingual Chinese/English folders…By the end of the congress all of the contact forms were gone“, so Mr. Ecker after the Congress.
Main focus of the TissueGnostics booth was TissueFAXS, HistoFAXS and HemoFAXS. HemoFAXS offers fully automated analysis and classification of Leukocytes (white blood cells) based on advanced Support Vector Machine Technology.
TissueFAXS is a cell and tissue imaging and analysis system for fluorescence analysis. HistoFAXS does the same in brightfield. Due to superb, patented algorithms TissueGnostics systems are in use all over the world.