TissueGnostics | TissueQuest

Image Analysis Software

Our team is highly competent in medical research and software development thanks to long years of experience. We are therefore able to meet specific needs by focusing on customer profiles. Our competence is also apparent from numerous publications in top journals, which have earned us a solid reputation in the international scientific community.

A vital element of our company policy is to provide for future needs through research and innovation. Finding ways to identify and represent cells inside their native tissue environment has been a key objective of our study groups over many years. We have devoted extensive research and development work to these issues. Our experience with immunohistochemical and fluorescent staining techniques is second to none. We therefore guarantee to our customers that we only sell top-quality solutions!

TissueGnostics solutions are breaking new ground in tissue analysis. We offer unique products of excellent quality that are convenient to use, and we offer comprehensive aftersale service.

Unique Design

TissueQuest is the first software solution worldwide to identify and analyze individual cells in their native tissue environment. It supports any number of fluorescent markers used for this purpose.

Cell identification is not confined to isolated cells. TissueQuest also identifies cells that are agglomerated in dense clusters or have been reduced to ring structures (as is typical of cancer cells).

With Tissue Quest, we offer our customers a unique diagnostic tool. This is the first time that tissue can be mapped and analyzed in a truly objective and reproducible manner.

Identifying individual cells

Selecting cellular staining patterns

Individual cells are identified by capturing distinctive staining patterns (such as nuclei, ring structures and cytoplasm). These patterns can be freely selected by the user. A dedicated master channel to identify nuclei is used for cell segmentation.

Example of automated Nuclear
identification

See example

Example of automated Cytoplasm identification

Beispiel zeigen

Analysis solutions

Individual cells
Individual cells in tissue
Individual cells with ring structures
Individual cells in clusters
Cytoplasm

Representation of Data

Identified cells are immediately displayed on screen as scattergrams, indicating surface area/DNA and staining intensity. Just like in flow cytometry, each dot illustrates one individual cell.

For further analysis, cutoff parameters need to be defined based on the negative control. Then gates are set to define which parameters will be analyzed.

Any cutoffs thus defined can be conveniently copied to the other scattergrams. Several gate settings within the same scattergram can be related to each other. Their shape and color can be individually readjusted.

Detail Window

Images and scattergrams can be enlarged in the detail view. This function is both convenient and offers highly effective overviews.

Effective Analysis

The results are immediately displayed after setting the gates. Calculated parameters include mean intensity, relative/absolute events, and percentage of reactive cells. The data are output in tabular format broken down into quadrants (UL-Upper Left, UR-Upper Right, LL-Lower Left, LR-Lower Right). In the past, this type of representation could not be modified by adjusting the parameters. With TissueQuest 2.0, however, any modifications made to the cutoffs and/or gates will promptly update the results.

Backward-Forward Gating

TissueQuest offers for the first time an innovative function for measurement control. Backward Gating from DotPlot to image and Forward gating from image to DotPlot.

Watch movie

Great Flexibility

TissueQuest can be used as stand alone application. All TissueQuest routines are fully automated and executed in real time. They open the door to prompt and observer-independent analysis.

TissueQuest - Cell recognition

Example of automated cell nucleus and cell cytoplasm identification

View example

Analysis using the TissueGnostics Staining Kit

Prostate cancer example Nr.1

TissueQuest Movie - Project overview

See how to:

Create a new Project
Import images
Set Mask Parameters
Set Cut-offs
Set Gates
Backward and Forward Gating from DotPlot to images and from images to DotPlot