TissueFAXS and TissueQuest
Immunofluorescence Cell Analysis System
The newly developed TissueFAXS is a unique instrument of cell analysis that combines detailed morphologic information offered by microscopy with the scientific accuracy of multi-channel flow cytometry.
- Fully automated microscope-based cell analysis system for immunofluorescence
- Autofocus up to 63x Oil Objective
- Multi-channel acquisition
- Automated identification of cells in tissue and smears
- Specimen overview and cellular detail in one interface
- Scatergramm and dot plot operations
- Forward Gating from the individual cell in the image to the dot in the scattergram as well as Backward Gating from the individual dot or gate to the corresponding cells in the sample
- Suitable for cryocut and paraffin sections, cell cultures, TMA's and smears
Workflow of the acquisition process
Unprecedented reliability in analyzing and diagnosing cells in tissue and smears
Overlay
DAPI
Mask
Cell identification
Scattergram
Statistics of scattergram and gates
Features of automated image acquisition:
Multi-channel acquisition
Improved overview by TissueStitching
Click on the image to enlarge
TissueFAXS is a high tech system offering automated routines for comprehensive procedures.
Still, its handling is remarkably simple. The analytical process is based on standardized image acquisition.
It is not linked to specific staining types or object sizes.
Examples of images acquired with the TissueFAXS System
Click on the image to enlarge
Click on the image to enlarge
Click on the image to enlarge
Click on the image to enlarge
TissueFAXS Hardware
Supported high-end microscopes:
AxioImager.Z1
AxioObserver.Z1
Nikon Eclipse 90i
Leica DM 6000
200 slides Prior loader
- Up to 7 objectives
- Up to 10 fluorescence filter sets
- High-precision scanning stage for up to 8 slides by Maerzhaeuser (Wetzlar, Germany).
- 200 slides loader by Prior (optional).
- High-performance monochrome camera for fluorescence microscopy by PCO (Kelheim, Germany).
- High-resolution colour camera for brightfield microscopy by PixeLink (Ottawa, Canada).
- High-performance PC, 2 TFT flat screens, 2 Gigabyte RAM, 1 Terabyte HD, Core 2 Duo Processor.
- The TissueFAXS hardware is assembled, programmed and tested by TissueGnostics (Vienna, Austria).
Software:
- TissueFAXS Software - Image Acquisition Software including TissueStitching function
- TissueQuest - Cell Analysing Software for Immunofluorescence
Attention: Consult operating instructions for use!
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