HistoFAXS and HistoQuest

Immunohistochemistry Cell Analysis System

HistoFAXS is a easy to use, microscopic analysis system that automatically acquires up to 8 slides with immunohistochemically stained sections and performs quantitative analysis of staining intensities.

• Automatic acquisition of an unlimited number of regions of interest on up to eight slides
• Possibility of large overview image export based on individual fields of view at user- definable resolutions
• Definition of regions to be analysed (or excluded from analysis) on the acquired regions of interest
• Semi-automated colour separation to extract the relevant marker information out of immunohistochemical images
• Reliable automatic nuclear segmentation with a minimum of user interaction
• Forward gating from the individual cell in the image to the dot in the scattergram as well as backward gating from the individual dot or dot group to the corresponding cells in the sample
• DotPlot operations – Re-evaluation and further analysis of the image processing results via gating and additional DotPlots

HistoFAXS provides a smooth, automated workflow from image acquisition to publication quality output of graphs and images as well as customizable data export for further processing.

Cutting-edge tool for cell-based analysis of staining intensities of immunohistochemically stained sections

Statistics of scattergram and gates

Tissue Micro Array (TMA)

Tissue Microarray (TMA) module is fully integrated in the HistoFAXS system. Acquisition is handled in the HistoFAXS software while the analysis is done in HistoQuest.

TMA spots are identified on a preview low magnification scan, acquisition can be made automatically, with manual adjustment or semi-manually by projecting TMA block patterns and doing comprehensive block operations. Each spot is given its individual ID on identification.

Logical groups can be drawn and named on the preview image, thus providing one possible basis for later analysis. After acquisition, the project is ready for analysis.

TMA Analysis

TMA projects are opened in HistoQuest.

Logical groups are shown in HistoQuests Input Region list and are analysed as one sample. TMA spots not in logical groups are displayed separately in this list under their spot ID. They can be analysed individually.

After analysis, results can be exported to Excel sheets to be linked with metadata for further treatment.

A browser-type TMA explorer and a report generator completes the TMA functionalities.

Features of automated image acquisition:

Fully automated slide scanning

Freely selectable areas of interest

Improved overview by TissueStitching

New standard for the entire process of slide scanning and data handling

HistoFAXS is a high tech system offering automated routines for comprehensive procedures.
Still, its handling is remarkably simple. The analytical process is based on standardized image acquisition.
It is not linked to specific staining types or object sizes.

Examples of images acquired with the HistoFAXS System

HistoFAXS Hardware

Supported high-end microscopes

AxioImager.Z1

AxioObserver.Z1

Nikon Eclipse 90i

Leica DM 6000

200 slides Prior loader

• Up to 7 objectives
• High-precision scanning stage for up to 8 slides by Maerzhaeuser (Wetzlar, Germany).
• High-high resolution colour camera for brightfield microscopy by PixeLink (Ottawa, Canada).
• High-performance PC, 2 TFT flat screens, 2 Gigabyte RAM, 1 Terabyte HD, Core 2 Duo Processor.
• The HistoFAXS hardware is assembled, programmed and tested by TissueGnostics (Vienna, Austria).

Software:

• HistoFAXS - Management and Acquisition Software
• HistoQuest - Tissue Analysis Software for Immunohistochemistry

Attention: Consult operating instructions for use!

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